Faculty

HYAM L. LEFFERT 

Professor of Pharmacology

Key Words: Gene Expression; Growth Factors; Liver Regeneration; Carcinogenesis; Gene Therapy; Stem Cells and Transplantation

Control of animal cell proliferation and differentiation. Liver regeneration: humoral control, protooncogenes, stem cells, transplantation.  Chemical hepatocarcinogenesis: carcinogen chemistry, stem cells, oncogenes, carcinogen targeting. Primary hepatocyte cell culture: growth factors, drug metabolism. Gene expression: microsatellites, gene transfer, gene targeting, unusual DNA structure.  Membrane ion fluxes: biochemistry, biophysics, molecular biology, monoclonal antibodies. 
 

Selected Publications:

Fabregat, I., Koch, K.S., Aoki, T., Atkinson, A.E., Dang, H., Amosova, O., Fresco, J.R., Schildkraut, C.L. and Leffert, H.L. Functional pleiotropy of an intramolecular triplex-forming fragment from the 3' UTR of the rat Pigr gene. Physiological Genomics 5: 53-65 (2001).

Zhang, M., Sell, S. and Leffert, H.L. Hepatic progenitor cell lines from allyl alcohol-treated adult rats are derived from gamma-irradiated mouse STO cells. Stem Cells 21: 449-458 (2003).

Koch, K.S., and Leffert, H.L. Normal liver progenitor cells in culture, in Stem Cells Handbook (ed. S. Sell), Humana Press, Inc., Totowa, NJ, pp. 367-384 (2004).  

Maeda, S., Chang, L., Li, Z-W., Luo, J-L., Leffert, H. and Karin, M. IKKß is required for prevention of apoptosis mediated by cell-bound but not by circulating TNF-alpha . Immunity (Cell) 19:725-737 (2003).

Leffert, H.L. and Sell, S. Letter to Editor. Unexpected artifacts raise new caution in stem cell culture research. Hepatology 39: 258 (2004).

Son, K-H., Zhang, M., Rucobo, E., Nwaigwe, D., Montgomery, F. and Leffert, H.L. Derivation and study of human epithelial cell lines resistant to killing by chromium trioxide. J. Toxic. Environ. Health Part A 67: 1027-1049 (2004).

Zhang, M., Joseph, B., Gupta, S., Guest, I., Xu, M., Sell, S., Son, K-H., Koch, K.S. and Leffert, H.L. Embryonic mouse STO cell-derived xenografts express hepatocytic functions in the livers of non-immunosuppressed adult rats. Stem Cells 23: 186-199 (2005).

Maeda, S., Kamata, H., Luo, J-L., Leffert, H. and Karin, M. IKKß couples hepatocyte death to cytokine-driven compensatory proliferation that promotes chemical hepatocarcinogenesis. Cell 121: 977-990 (2005).

Koch, K.S., Son, K.H., Maehr, R., Pellicciotta, I., Ploegh, H.L.,
Zanetti, M., Sell, S. and Leffert, H.L. Immune-privileged embryonic Swiss mouse STO and STO cell-derived progenitor cells: major histocompatibility complex and cell differentiation antigen expression patterns resemble those
of human embryonic stem cell lines. Immunology. 119:98-115 (2006)