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ROGER
Y. TSIENProfessor of Pharmacology and Chemistry & Biochemistry TEL: 858-534-4891FAX: 858-534-5270 email: rtsien@ucsd.edu
Key Words: Signal Transduction; Calcium; Neurobiology; Regulation and role of messengers such as calcium, cyclic AMP, and nitric oxide.
Engineering and use of new molecules to measure and perturb these messengers. Cellular mechanisms of synaptic plasticity, especially in cerebellum. Stimulus- transcription coupling via cAMP-dependent protein kinase. Mechanism of Ca2+ influx stimulated by emptying of endoplasmic reticulum stores. Structure-function relationships in Green Fluorescent Protein, and its application to monitor protein-protein interactions by fluorescence resonance energy transfer. Selected Publications: Zlokarnik, G., Negulescu, P.A., Knapp, T.E., Mere, L., Burres, N., Feng, L., Whitney, M., Roemer, K., and Tsien, R.Y. 1998. Quantitation of transcription and clonal selection of single living cells using beta-lactamase as reporter. Science 279: 84-88. Li, W., Llopis, J., Whitney, M., Zlokarnik, G., and Tsien, R.Y. 1998. Cell-permeant caged InsP3 ester shows that Ca2+ spike frequency can optimize gene expression. Nature 392: 936-941. Griffin, B.A., Adams, S.R., and Tsien, R.Y. 1998. Specific covalent labeling of recombinant protein molecules inside live cells. Science 281: 269-272. Miyawaki, A., Griesbeck, O., Heim, R., and Tsien, R.Y. 1999. Dynamic and quantitative Ca2+ measurements using improved cameleons. Proc. Natl. Acad. Sci. USA 96: 2135-2140. Yao, Y., Ferrer-Montiel, A.V., Montal, M., and Tsien, R.Y. 1999. Activation
of store-operated Ca2+ current in xenopus oocytes requires
SNAP-25 but not a diffusible messenger. Cell, in press, August 20. |
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Main address: Department of Pharmacology, University of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093-0636 pharmhr@ucsd.edu Terms and Conditions of Use Copyright ©2003 Regents of the University of California. All rights reserved. |