Professor of Pharmacology and Chemistry & Biochemistry
Investigator, Howard Hughes Medical Institute
Ph.D. Unviersity of Cambridge, England
Key Words: Signal Transduction; Calcium; Neurobiology
Protease-directed molecular targeting of contrast agents and drugs to tumors, atherosclerotic plaques, strokes, and arthritic joints. Delivery of hydrophilic drugs into the cytosol and across the blood-brain barrier. Fluorescence highlighting of myelin for image-guided surgery. Imaging newly synthesized proteins during synaptic plasticity and neuropathology. Genetically encoded transducers to stimulate neuronal fi ring in response to light or magnetic fi elds. Infrared-fl uorescent proteins for imaging cell and molecular dynamics in thick tissues and organisms. Singlet-oxygen-generating proteins for electron microscopy, acute inactivation of proteins, and proximity sensing.
Xiaokun Shu, Varda Lev-Ram, Thomas J. Deerinck, Yingchuan Qi, Ericka B. Ramko, Michael W. Davidson, Yishi Jin, Mark H. Ellisman, Roger Y. Tsien 2001. A Genetically Encoded Tag for Correlated Light and Electron Microscopy of Intact Cells, Tissues, and Organisms. Plosbiology, 9:1-10
Emilia S. Olson, Tao Jiang, Todd A. Aguilera, Quyen T. Nguyen, Lesley G. Ellies, Miriam Scadeng, and Roger Y. Tsien 2010 Activatable cell penetrating peptides linked to nanoparticles as dual probes for in vivo fl uorescence and MR imaging of proteases. Proc. Natl. Acad. Sci. 107 : 4311–4316
Quyen T. Nguyen, Emilia S. Olson, Todd A. Aguilera, Tao Jiang, Miriam Scadeng, Lesley G. Ellies,and Roger Y. Tsien 2010 Surgery with molecular fl uorescence imaging using activatable cell-penetrating peptides decreases residual cancer and improves survival. Proc. Natl. Acad. Sci. 107 : 4317–4322
Michael A Whitney, Jessica L Crisp, Linda T Nguyen, Beth Friedman, Larry A Gross, Paul Steinbach, Roger Y Tsien & Quyen T Nguyen 2011. Fluorescent peptides highlight peripheral nerves during surgery in mice. Nature Biotech: Advance Online Publication
Margaret T. Butko, Jin Yang, Yang Geng, Hyung Joon Kim, Noo Li Jeon, Xiaokun Shu, Mason R. Mackey, Mark H. Ellisman, Roger Y. Tsien and Michael Z. Lin. Fluorescent and photo-oxidizing TimeSTAMP tags track protein fates in light and electron microscopy. Nat Neurosci. 2012 December; 15(12): 1742–1751.