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Roger Tsien
Professor of Pharmacology and Chemistry & Biochemistry
Phone: 858-534-4891
Fax: 858-534-5270
E-mail: rtsien@ucsd.edu
Lab Website
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Key Words: Signal Transduction; Calcium; Neurobiology
Engineering and use of new molecules to measure and perturb these messengers. Cellular mechanisms of synaptic plasticity, especially in cerebellum. Stimulus- transcription coupling via cAMP-dependent protein kinase. Mechanism of Ca2+ influx stimulated by emptying of endoplasmic reticulum stores. Structure-function relationships in Green Fluorescent Protein, and its application to monitor protein-protein interactions by fluorescence resonance energy transfer.
Recent Publications
Lin, M.Z., Glenn, J.S., and Tsien, R.Y. 2008. A drug-controllable tag for visualizing newly synthesized proteins in cells and whole animals. Proc. Natl. Acad. Sci. USA 105: 7744-7749.
Shu, X., Royant, A., Lin, M.Z., Aguilera, T.A., Lev-Ram, V., Steinbach, P.A., and Tsien, R.Y. 2009. Mammalian expression of infrared fluorescent proteins engineered from a bacterial phytochrome. Science 324: 804-807.
Olson, E.S., Jiang, T., Aguilera, T.A., Nguyen, Q.T., Ellies, L.G., Scadeng, M., and Tsien, R.Y. 2010. Activatable cell penetrating peptides linked to nanoparticles as dual probes for in vivo fluorescence and MR imaging of proteases. Proc. Natl. Acad. Sci. USA 107: 4311-4316.
Nguyen, Q.T., Olson, E.S., Aguilera, T.A., Jiang, T., Scadeng, M., Ellies, L.G., and Tsien, R.Y. 2010. Surgery with molecular fluorescence imaging using activatable cell-penetrating peptides decreases residual cancer and improves survival. Proc. Natl. Acad. Sci. USA 107: 4317-4322.
Whitney, M., Crisp, J.L., Olson, E.S., Aguilera, T.A., Gross, L.A., Ellies, L.G., and Tsien, R.Y. 2010. Parallel in vivo and in vitro selection using phage display identifies protease dependent tumor targeting peptides. J. Biol. Chem. 285: 22532-22541
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